1.  Rip the aluminum foil pouch to take out the cassette, and put it on a clean place.

2.  Specimen collection: according to standard operating methods.

3.  Extraction specimen: Unscrew an extraction tube tip and insert the swab into the extraction reagent tube. While squeezing the reagent tube, stir the swab more than 5 times. 

4.  Remove the swab while squeezing the sides of the tube to extract the liquid from the swab.

5. Press the extraction tube tip tightly onto the tube. 

6.  Hold the extraction tube with extraction tube tip above the test cassette. Apply 3 drops of extracted specimen to the sample well of the cassette, respectively and then start the timer.

7.  Observe the results in 15 minutes. The test is invalid clinically after 20 minutes.